eISSN: 2353-9461
ISSN: 0860-7796
BioTechnologia
Current issue Archive About the journal Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures
Editorial System
Submit your Manuscript
SCImago Journal & Country Rank
2/2020
vol. 101
 
Share:
Share:
abstract:
RESEARCH PAPERS

Value addition to Pongamia biodiesel industry through bioethanol production from pressed oil cake using Bacillus cereus

Kamath H. Venkatesh
1
,
Vaman C. Rao
1

  1. Department of Biotechnology Engineering, N.M.A.M. Institute of Technology, (Visvesvaraya Technological University), Belagavi, Nitte, Karnataka, India
BioTechnologia vol. 101 (2) C pp. 147–158
Online publish date: 2020/06/16
View full text Get citation
 
PlumX metrics:
Pongamia pinnata, a tree bearing oil seeds, has been one of the prime focus among nonedible oil sources for biodiesel production in most parts of India. The oil cake obtained after the extraction of oil serves as an adequate source of cellulose, hemicellulose, proteins, lipids, and starch. The scarce literature data available suggest very low conversion rate of complex sugars to fermentable sugars and further to bioethanol. In the present work, a sulfuric acid hydrolysate obtained from Pongamia oil cake (POC) under microwave irradiation was anaerobically fermented using Bacillus cereus ATCC 14579. Under anaerobic and acidic conditions, the process parameters affecting the bioethanol production efficiency (BPE) were screened and optimized using statistical experimental designs. Of the seven parameters screened (glucose concentration, yeast extract as nitrogen source, agitation speed, pH, inoculum size, fermentation time, and temperature), only 3 were found to be significant and to increase BPE. The significant parameters were optimized using the Box-Behnken experimental design to obtain an optimal condition of 9 g/l reducing sugars, pH 4.6 maintained with acetate buffer, and fermentation period of 51 h that yielded 92% BPE with 4.2 g/l ethanol. Moreover, it was observed that the use of a buffer system to maintain broth pH, especially acetate buffer, significantly improved BPE. Low concentration of fermentation inhibitors is the key factor for improved BPE. The research outcome suggests that the production of dual fuel from Pongamia pinnata, viz. biodiesel and bioethanol, could not only improve the process economics but also reduce waste generation.
keywords:

Pongamia pinnata, oil cake hydrolysate, bioethanol, Bacillus cereus, value addition



Stosujemy się do standardu HONcode dla wiarygodnej informacji zdrowotnej This site complies with the HONcode standard for trustworthy health information: verify here